scRNA-seq Labeling Models

CellTypist Labeling Models

To enable you to label your own PBMC datasets, we supply models for the CellTypist cell labeling framework. Below are links for each of the 3 cell type resolutions generated for our Healthy Immune Atlas:

Level 1 (AIFI_L1): 9 broad cell classes
Level 2 (AIFI_L2): 29 intermediate resolution cell types
Level 3 (AIFI_L3): 71 high resolution cell types

Here, we provide models that use the full set of 10x Genomics 3' scRNA-seq features, as well as models for use with data generated using the 10x Genomics Single Cell Gene Expression Flex scRNA-seq system, which uses a subset of the genes available for 3' scRNA-seq.

Immune Health Atlas 10x 3' CellTypist Models
File NameDescriptionDownload Link
ref_pbmc_clean_celltypist_model_AIFI_L1_2024-04-18.pkl Broad cell class labeling model for CellTypist
ref_pbmc_clean_celltypist_model_AIFI_L2_2024-04-19.pkl Intermediate cell type labeling model for CellTypist
ref_pbmc_clean_celltypist_model_AIFI_L3_2024-04-19.pkl High resolution cell type labeling model for CellTypist
Immune Health Atlas 10x Flex CellTypist Models
File NameDescriptionDownload Link
ref_pbmc_clean_celltypist_model_flex-features_AIFI_L1_2024-04-18.pkl
ref_pbmc_clean_celltypist_model_flex-features_AIFI_L2_2024-04-19.pkl
ref_pbmc_clean_celltypist_model_flex-features_AIFI_L3_2024-04-19.pkl

Cell Type Colorset

In addition to our labeling models, we provide the colorsets that we used to generate our scRNA-seq visualizations. These colors were selected in collaboration with Heidi Gustafson, an artist and pigment foraging expert. More information about Heidi's work can be found at https://earlyfutures.com/.

Immune Health Atlas Colorsets
File NameDescriptionDownload Link
AIFI_L1_imm_health_atlas_type_order_colors.csv
AIFI_L2_imm_health_atlas_type_order_colors.csv
AIFI_L3_imm_health_atlas_type_order_colors.csv
AIFI_imm_health_atlas_type_order_colors.csv