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    • Plasma Proteomics

    Olink Plasma Proteomics Data

    Relative plasma protein abundance was measured using the Olink Explore 1536 platform in multiple batches. To normalize across batches, we used a common set of samples included in each batch, and used these common samples to calculate batch normalization offsets. The offsets learned from the batch controls were then applied across all assays in each batch.

    Our Olink datasets include the original normalized protein expression (NPX) and limit of detection (LOD) values reported by Olink, as well as these offset values and batch corrected NPX and LOD.

    The plasma proteomics dataset contains some sample-level metadata to enable connection between these results and samples used in other modalities. The sample.sampleKitGuid identifier is used across many data modalities:

    Sample Identifiers
    sample.sampleKitGuid: A Globally Unique Identifier (GUID) for the Sample Kit, representing all material collected at a visit
    specimen.specimenGuid: A GUID for the Specimen, representing the specific aliquot of plasma that was measured

    Olink Data
    olink.batch_id: A unique ID specifying each batch of samples submitted together
    olink.plate_id: A unique ID for the specific plate the samples were aliquoted into
    olink.panel: The name of the panel of Olink assays (Cardiometabolic, Inflammation, Oncology, or Neurology)
    olink.assay: Protein name/symbol for the specific Olink assay (e.g. TNFRSF1B, IL7R)
    olink.assay_id: A unique ID for the specific Olink assay (e.g. OID21145, OID21136)
    olink.uniprot_id: The Uniprot ID for the protein assayed (e.g. P20333, P16871)
    olink.norm_offset: The offset value used to normalize between batches
    olink.NPX_raw: The normalized protein expression (NPX) value reported by Olink
    olink.NPX_norm: The batch-normalized NPX value after adjusting with the olink.norm_offset value
    olink.LOD_raw: The limit of detection (LOD) value reported by Olink
    olink.LOD_norm: The batch-normalized LOD value after adjusting with the olink.norm_offset value

    Subject Identifiers and Metadata (prefix: cohort or subject)
    cohort.cohortGuid: A GUID for the Cohort the subject enrolled in for our study
    subject.subjectGuid: A GUID for the Subject
    subject.biologicalSex: The biological sex of the Subject
    subject.birthYear: The Birth Year of the Subject
    subject.ageGroup: The Age Group that the subject belongs to (Pediatric, Young Adult, or Older Adult)
    subject.race: The self-reported Race of the Subject
    subject.ethnicity: The self-reported Ethnicity of the subject
    subject.cmv: The CMV status of the subject (Positive or Negative)

    Sample Metadata (prefix: sample)
    sample.visitName: The name of the study visit (i.e. time point)
    sample.subjectAgeYears: The age of the Subject in years at the time of sample collection

    Human Immune Health Atlas Olink Data
    File Name Description Download Link
    human_immune_health_atlas_olink.csv Olink data from all samples